|
KMID : 0903519760190030162
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1976 Volume.19 No. 3 p.162 ~ p.171
|
|
|
Kinetic Analysis of Rate Limiting Step in the Metabolic Process
|
|
|
|
|
Abstract
|
|
|
|
The determination of rate limiting step in the metabolic process is of great importance to understand the metabolic properties. In this paper the authors propose a modified enzymatic method instead of thermodynamic method. This method is based on the assumption that the over-all rate increment would be larger than by any other steps to which the individual enzyme are added respectively, provided that the enzyme participated in the rate limiting step is added to the reactions composed of n steps of metabolic process with which n kind of enzymes are concerned. The present paper deals with analysis and discussion about some factors having influence on the proposed process, mainly about the metabolic process constituted with homogeneous steps. The results show that the determination of rate limiting step by a modified enzymatic method is feasible, provided that some restrictions arc added in any type of mechanisms.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|